DNA Technology and Transgenic Organisms
About this chapter
DNA Technology and Transgenic Organisms - Chapter Summary and Learning Objectives
DNA is the building block of life. If you watch those television crime dramas, you've probably heard of DNA being used to catch criminals. It is actually fascinating and does much more than allow cops to catch the bad guys. In this chapter, instructor, Elizabeth Wood, will help you understand more about DNA and related concepts. You'll see how polymerase can amplify DNA, learn what a microarray is, and study advances in DNA technology. Additional things that will be covered in these lessons:
- The polymerase chain reaction
- DNA in cloning
- Gene analysis
- DNA sequences
- Genetic modification of food and animals
- How genetic modification helps livestock
|PCR: Reagents Used in Polymerase Chain Reaction||Learn the steps of the polymerase chain reaction.|
|Recombinant DNA: Cloning, Restriction Enzymes, and Plasmids||Discover the basics of recombinant DNA technology.|
|Microarrays: Definition & Analyzing Genes on a Chip||Examine the basics of microarray for analyzing gene-expression.|
|DNA Sequencing and the Human Genome||Explore how DNA sequences are generated.|
|siRNA||See how siRNA inhibits translation of complementary mRNA.|
|Introduction to Genetically Modified Organisms (GMOs): Synthesizing Recombinant Insulin||Study GMOs and synthetic insulin production.|
|GMOs: Improving Nutrition and Golden Rice||Find out why golden rice is created.|
|GMOs: Growing Herbicide-Resistant Crops||Explore how herbicide-resistant crops are produced.|
|GMOs: Producing Infection-Resistant Cows||Analyze why genetic modification is helpful in cows.|
How do we make the insulin used by diabetic patients? In this lesson, you'll learn the basics of how genetic engineering can be used to transform a bacterial host cell into a genetically-modified organism that produces human insulin.
DNA plasmids play an integral part in most genetic engineering experiments. In this lesson, you'll learn about key features of a plasmid, such as a multiple cloning site, an origin of replication, and a selectable marker.
Restriction enzymes played a critical role in the advent of genetic engineering. In this lesson, you will learn what role restriction enzymes play in creating recombinant DNA.
DNA ligase makes recombinant DNA technology possible. In this lesson, you will learn how new versions of genes can be designed for experiments in novel host organisms using DNA ligase.
The ability to determine the DNA sequence of an individual is a powerful tool for paternity questions and criminal investigations, among other uses. This lesson will describe one laboratory method that can be used to sequence DNA.